GlycanGUI: Automated Glycan Annotation and Quantification Using Glucose Unit Index

The retention time provides critical information for glycan annotation and quantification from the Liquid Chromatography Mass Spectrometry (LC-MS) data. However, variation of precise glycans is highly dependent on experimental conditions such as specific separating columns, MS instruments and/or buffer used. This hampers exploitation LC-MS data, especially when inter-laboratory data are compared. To incorporate across experiments, Glucose Unit Index (GUI) can be computed using dextrin ladder internal standard. then calibrated with respect to glucose units derived ladders. Despite successful application GUI approach, manual calibration process quite tedious often error prone. In this work, we present a standalone software tool GlycanGUI, graphic user interface automatically carry out GUI-based annotation/quantification subsequent analysis. When tested GlycanGUI reported accurate values compared calibration, thus ready used automated GUI.